1,897 research outputs found
Evaluating the ecological realism of plant species distribution models with ecological indicator values
Species distribution models (SDMs) are routinely applied to assess current as well as future species distributions, for example to assess impacts of future environmental change on biodiversity or to underpin conservation planning. It has been repeatedly emphasized that SDMs should be evaluated based not only on their goodness of fit to the data, but also on the realism of the modelled ecological responses. However, possibilities for the latter are hampered by limited knowledge on the true responses as well as a lack of quantitative evaluation methods. Here we compared modelled niche optima obtained from European-scale SDMs of 1,476 terrestrial vascular plant species with empirical ecological indicator values indicating the preferences of plant species for key environmental conditions. For each plant species we first fitted an ensemble SDM including three modeling techniques (GLM, GAM and BRT) and extracted niche optima for climate, soil, land use and nitrogen deposition variables with a large explanatory power for the occurrence of that species. We then compared these SDM-derived niche optima with the ecological indicator values by means of bivariate correlation analysis. We found weak to moderate correlations in the expected direction between the SDM-derived niche optima and ecological indicator values. The strongest correlation occurred between the modelled optima for growing degree days and the ecological indicator values for temperature. Correlations were weaker for SDM-derived niche optima with a more distal relationship to ecological indicator values (notably precipitation and soil moisture). Further, correlations were consistently highest for BRT, followed by GLM and GAM. Our method gives insight into the ecological realism of modelled niche optima and projected core habitats and can be used to improve SDMs by making a more informed selection of environmental variables and modeling techniques
Counting maximal arithmetic subgroups
We study the growth rate of the number of maximal arithmetic subgroups of bounded covolumes in a semisimple Lie group using an extension of the method developed by Borel and Prasad
Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT
The nuclear pore complex (NPC) is one of the largest and most complex protein assemblies in the cell and, among other functions, serves as the gatekeeper of nucleocytoplasmic transport. Unraveling its molecular architecture and functioning has been an active research topic for decades with recent cryogenic electron microscopy and super-resolution studies advancing our understanding of the architecture of the NPC complex. However, the specific and direct visualization of single copies of NPC proteins is thus far elusive. Herein, we combine genetically-encoded self-labeling enzymes such as SNAP-tag and HaloTag with DNA-PAINT microscopy. We resolve single copies of nucleoporins in the human Y-complex in three dimensions with a precision of circa 3 nm, enabling studies of multicomponent complexes on the level of single proteins in cells using optical fluorescence microscopy
Determining cellular CTCF and cohesin abundances to constrain 3D genome models.
Achieving a quantitative and predictive understanding of 3D genome architecture remains a major challenge, as it requires quantitative measurements of the key proteins involved. Here, we report the quantification of CTCF and cohesin, two causal regulators of topologically associating domains (TADs) in mammalian cells. Extending our previous imaging studies (Hansen et al., 2017), we estimate bounds on the density of putatively DNA loop-extruding cohesin complexes and CTCF binding site occupancy. Furthermore, co-immunoprecipitation studies of an endogenously tagged subunit (Rad21) suggest the presence of cohesin dimers and/or oligomers. Finally, based on our cell lines with accurately measured protein abundances, we report a method to conveniently determine the number of molecules of any Halo-tagged protein in the cell. We anticipate that our results and the established tool for measuring cellular protein abundances will advance a more quantitative understanding of 3D genome organization, and facilitate protein quantification, key to comprehend diverse biological processes
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3ânm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells
The cellular microscopy phenotype ontology
BACKGROUND:
Phenotypic data derived from high content screening is currently annotated using free-text, thus preventing the integration of independent datasets, including those generated in different biological domains, such as cell lines, mouse and human tissues.
DESCRIPTION:
We present the Cellular Microscopy Phenotype Ontology (CMPO), a species neutral ontology for describing phenotypic observations relating to the whole cell, cellular components, cellular processes and cell populations. CMPO is compatible with related ontology efforts, allowing for future cross-species integration of phenotypic data. CMPO was developed following a curator-driven approach where phenotype data were annotated by expert biologists following the Entity-Quality (EQ) pattern. These EQs were subsequently transformed into new CMPO terms following an established post composition process.
CONCLUSION:
CMPO is currently being utilized to annotate phenotypes associated with high content screening datasets stored in several image repositories including the Image Data Repository (IDR), MitoSys project database and the Cellular Phenotype Database to facilitate data browsing and discoverability
Using species attributes to characterize late-glacial and early-Holocene environments at KrÄkenes, western Norway
Aim: We aim to use species attributes such as distributions and indicator values to reconstruct past biomes, environment, and temperatures from detailed plantâmacrofossil data covering the late glacial to the early Holocene (ca. 14â9 ka). Location: KrĂ„kenes, western Norway. Methods: We applied attributes for presentâday geographical distribution, optimal July and January temperatures, and Ellenberg indicator values for plants in the macrofossil dataâset. We used assemblage weighted means (AWM) to reconstruct past biomes, changes in light (L), nitrogen (N), moisture (F), and soil reaction (R), and temperatures. We compared the temperature reconstructions with previous chironomidâinferred temperatures. Results: After the start of the Holocene around 11.5 ka, the Arcticâmontane biome, which was stable during the lateâglacial period, shifted successively into the Boreoâarctic montane, Wideâboreal, Boreoâmontane, Boreoâtemperate, and Wideâtemperate biomes by ca. 9.0 ka. Circumpolar and Eurasian floristic elements characteristic of the lateâglacial decreased and the Eurosiberian element became prominent. Light demand (L), soil moisture (F), nitrogen (N), and soil reaction (R) show different, but complementary responses. Lightâdemanding plants decreased with time. Soil moisture was relatively stable until it increased during organic soil development during the early Holocene. Soil nitrogen increased during the early Holocene. Soil reaction (pH) decreased during the AllerĂžd, but increased during the Younger Dryas. It decreased markedly after the start of the Holocene, reaching low but stable levels in the early Holocene. Mean July and January temperatures show similar patterns to the chironomidâinferred mean July temperature trends at KrĂ„kenes, but chironomids show larger fluctuations and interesting differences in timing. Conclusion: Assigning attributes to macrofossil species is a useful new approach in palaeoecology. It can demonstrate changes in biomes, ecological conditions, and temperatures. The lateâglacial to earlyâHolocene transition may form an analogue for changes observed in the modern arctic and in mountains, with melting glaciers, permafrost thaw, and shrub encroachment into tundra.publishedVersio
Communication
Visualizing the functional interactions of biomolecules such as proteins and nucleic acids is key to understanding cellular life on the molecular scale. Spatial proximity is often used as a proxy for the direct interaction of biomolecules. However, current techniques to visualize spatial proximity are either limited by spatial resolution, dynamic range, or lack of single-molecule sensitivity. Here, we introduce Proximity-PAINT (pPAINT), a variation of the super-resolution microscopy technique DNA-PAINT. pPAINT uses a split-docking-site configuration to detect spatial proximity with high sensitivity, low false-positive rates, and tunable detection distances. We benchmark and optimize pPAINT using designer DNA nanostructures and demonstrate its cellular applicability by visualizing the spatial proximity of alpha- and beta-tubulin in microtubules using super-resolution detection. © 2020 Wiley-VCH GmbH
Protecting Clinical Trial Participants and Protecting Data Integrity: Are We Meeting the Challenges?
Susan Ellenberg discusses alternative approaches towards evaluating data as it accumulates in clinical trials, and to protecting the integrity and preventing undue risks to participants, as the trial continues
Testing Hardy nonlocality proof with genuine energy-time entanglement
We show two experimental realizations of Hardy ladder test of quantum
nonlocality using energy-time correlated photons, following the scheme proposed
by A. Cabello \emph{et al.} [Phys. Rev. Lett. \textbf{102}, 040401 (2009)].
Unlike, previous energy-time Bell experiments, these tests require precise
tailored nonmaximally entangled states. One of them is equivalent to the
two-setting two-outcome Bell test requiring a minimum detection efficiency. The
reported experiments are still affected by the locality and detection
loopholes, but are free of the post-selection loophole of previous energy-time
and time-bin Bell tests.Comment: 5 pages, revtex4, 6 figure
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